Charak Samhita

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रसात्ममातापितृसम्भवानि भूतानि विद्याद्दश षट् च देहे। चत्वारि तत्रात्मनि संश्रितानि स्थितस्तथाऽऽत्मा च चतुर्षु तेषु ॥३३॥

In the body, the bhutas are sixteen (each set of four bhutas is of four types), originating from maternal nutrition, the self, mother (ovum) and father (sperm). Out of these, four are associated with the self and the self, in turn, depends on these four.

english translation